Listado de Cambio

Nombre	Replicación	Dimensión	Elemento afectado	Situación en el estudio base	Situación en la replicación	Propósitos
Increase in the number of pots per treatment type	Soil-2018	Protocolo	Diseño experimental	There were (3x2x2x2) 24 experimental units (3 Cu levels, 2 JBR, 2 plant types and 2 soil types). For each experimental unit a pot is prepared and repeated 3 times.	There are (4x2) 8 experimental units (4 Cu and 2 JBR levels). For each experimental unit 4 pots are prepared and placed in a tray. This is repeated 3 times.	Increase the number of pots to obtain sufficient biomass.
Biomass obtained at fruiting stage	Soil-2018	Operacionalización	Procedimientos de medida	Biomass was collected when plants had 2 to 3 true leaves.	Biomass was collected when plants reached the fruiting stage.	Obtain more biomass as plants complete their vegetative cycle.
Increasing the volume of the pots	Soil-2018	Protocolo	Material experimental	The pots were of the 300 ml tube type.	The pots were of the 500 ml bucket type.	Achieve greater root development and produce more biomass.
Growing in greenhouses	Soil-2019	Contexto	Medium	The experiment was carried out in a culture chamber	The experiment was carried out in a greenhouse	Simulate natural conditions.
Mustard is only grown	Soil-2019	Protocolo	Instrumentos de medida	Two plants were used: barley and mustard.	Only the mustard plant was used.	Use the plant with the highest Cu phytoextraction capacity.
Naturally contaminated soils	Soil-2019	Operacionalización	Tratamiento	Two soil types were sampled: Coria (pH=7.8) and Constantina (pH=5.5).	Three soil types were sampled: Miraflores-1 and Miraflores-2 (with Pb, Zn and Cu) and Lebrija (not contaminated by metals)	Use naturally contaminated soils. The Miraflores soils are urban gardens with natural contamination and the Lebrija soil is used as a control.
Cu null dose	Soil-2019	Operacionalización	Tratamiento	The doses of Cu added to the soils were 0, 500 and 1000 mg/kg	Soils are not artificially contaminated with Cu	Experiment with the Cu already in the soil
Number of pots per treatment type	Soil-2019	Protocolo	Diseño experimental	There were (3x2x2x2) 24 experimental units (3 Cu levels, 2 JBR, 2 plant types and 2 soil types). For each experimental unit a pot is prepared and repeated 3 times.	There are (3x2) 6 experimental units (3 soils and 2 JBR levels). For each experimental unit 4 pots are prepared forming a block. This is repeated 3 times.	Analyse the influence of two naturally contaminated soils and a third uncontaminated one.
biomass at fruiting phase	Soil-2019	Operacionalización	Procedimientos de medida	Biomass was collected when plants had 2 to 3 true leaves.	Biomass was collected when plants reached the fruiting stage.	Obtain more biomass as plants complete their vegetative cycle.
Increase the volume of pots	Soil-2019	Protocolo	Material experimental	The pots were of the 300 ml tube type.	The pots were of the 500 ml bucket type.	Achieve greater root development and produce more biomass.